The importance of serological tests in the maintenance of laying hens

My Dad

Kediri-East Java

How important are serological tests in the maintenance of laying hens? What laboratory tests can be performed during the maintenance period?

Answer:

Dear. Mr. Sandi, thank you for the question. As we already know, the rise of viral diseases that infect chickens today requires a strict vaccination and biosecurity program. In addition to this, it is also recommended to monitor (monitor) on the health status of the chicken is to perform serological tests.

Serology test is defined as a test performed to see a picture of antibody titer in the body of chickens. This test technique uses blood serum as a sample, because it contains antibodies (humoral antibodies, red). Broadly speaking, the benefits of serology test there are 2, namely monitor health status and confirmation of diagnosis. Monitoring health status is synonymous with prevention efforts. Monitoring these include:

• Measuring maternal antibodies measurement of maternal antibodies through serological tests is often done to determine the Gumboro vaccination schedule. Thus, the condition of the decrease in maternal antibody titer will be predicted so that it can be estimated the right time for the first Gumboro vaccination and the selection of the vaccine type.
• See the success of vaccinationto see the success of vaccination is to see the formation of humoral antibodies (antibodies circulating in the blood) with serological tests. This serological test can be done 2-3 weeks post vaccination using active vaccine, or 3-4 weeks post vaccination using inactivated vaccines. Measurement of humoral antibodies will provide a representative picture when performed on chickens that already have a perfect humoral immune formation system that is > 5 weeks old.
• Determine the schedule of revaccination (revaccination)if you want to do monitor titer routinely during production, serology tests can be done every 1-2 months so that the protective antibody titer picture will continue to be monitored and it can be estimated the right time for chickens to be vaccinated again.
• Early warning systemSystem early warning it can run if we have data baseline titers from chickens raised and obtained through serological tests routinely every month. Baseline titer is the minimum level of antibody titer so that chickens are safe from certain disease infections.
• Some types of diseases that usually attack laying hens such as ND, AI and IB are sometimes difficult to distinguish if they do not show typical symptoms. In these conditions serological tests need to be done to help confirm the diagnosis. Sampling for confirmation of this diagnosis is done 2 times at the beginning of the case and 12-21 days later. The presence of a 4-fold increase in titer and non-uniform titer picture on the second examination, it can be concluded that most likely the chicken is infected with a field virus in accordance with the type of antibody tested.

There are several types of methods used in serology tests, some of which are Haemagglutination Inhibition test (HI test), Enzyme-linked Immunosorbent Assay (ELISA), Rapid Plate Agglutination test (RPAT), Agar Gel Precipitation test (AGPT), and Serum Neutralization test (SNT). Serology test is most often the choice for farmers is the HI method test and Elisha. In addition to this, we also offer a comprehensive range of laboratory equipment, including: laboratory equipment, laboratory equipment, laboratory equipment, laboratory equipment, laboratory equipment (MediLab) which also provides serological testing services, both HI test and Elisha.

In addition to serological tests, it is also necessary to regularly test the quality of the ration. During the period of keeping laying hens, the usual ration can be given in the form of a finished ration or ration self mixing. Ration quality test is important especially for the use of rations self mixing, considering that the formulation is done alone so that the percentage of use must adjust to the nutritional content of raw materials used. As for the finished ration, the ration quality test can be done in order to test the ration-monitor nutrient content of the ration during storage. Therefore, the presence of MediLab ready to help provide reliable ration test services.

Greetings.